The end of my first week in Vet School (Day 11)

Staining the mouse testes in acetic-orcein

Today was Friday, and was probably one of the lectures I’d been dreading most as the older years have said that this professor loves to fail people… So I headed to genetics and prayed that he wouldn’t pick me to fail, todays lecture was an introduction followed by looking at Mitosis (creating a replica of the cell (diploid as two sets of chromosomes)) and Meiosis (creating germ cell (haploid as one set of chromosomes)) in depth with and the interactions of this on  the chromosomes. I guess this kinda was revision, however I am going need to spend a couple hours here with my own book to get the processes spot on as I have not done it in a while.

This was then followed by the practical for Milk Hygiene, during which this week was mainly a recap of the previous lecture we had. We did however discover the power of questions as in shops here they seperate what we have always assumed to be milk products into two sections. Basically with milk products sometimes to reduce the cost producers replace milk cream with vegetable oil which then removes the product from the classifcation of milk products. So basically in the stores here there are milk products and then products that include milk. The rest of the lecture was on hygiene and process of hygiene and laboratory testing during milk production.

Note: Whilst I do have pictures of the dissection, I have made the decision not to include them within my diary as I want it to appeal to as wider range of readers as possible.

Our timetable got changed again leaving us with a 2 hour gap so we then all headed back to dorms, I did an hours studying and took a 30 minute nap. Also my bottle of water had leaked in my bag so put stuff out to dry. I then headed back to my Genetics practical with just my labcoat, notebook and genetics book. On sitting down the lab tech walked out and put a dead mouse in front of us, and was like collect the testes. I’ve never done anything with mice before so had no clue as to the anatomy of the urinary tract. Pulling a glove onto my left hand as I had a open wound I set about exploring my mouses abdomen with scalpel and scissors. I was very conscious about not damaging any internal structures but with limited dissection experience and no clue as to the anatomy was a little nervous. I opened to skin layer exposing the peritoneum (basically a sack that surrounds the abdomen organs). Now I had already admitted to knowing nothing about mouse anatomy so this was kinda cool, and I then opened the peritoneum exposing the organs. Now in relation to the size of the penis, the testes were large about the size of small marbles which suprised me a little. I harvested one and passed the mouse onto the next group for the other. The teste was then cut up into very small pieces and stained in acetic-orcein (which is a red stain) before being examined under the microscope.

Staining the mouse testes in acetic-orceinThe cells were then examined under the microscope to look for germ cells in different stages of maturity before they actually formed sperm cells. I am awful at recognising things under the microscope from paper (especially when it is black and white), however others in the class managed and I looked at the cells under their microscopes before then finding it with my own. We then also examined a onion root to look at the different stages of mitosis.

On the way out as the campus was so quite I decided to get a picture with Ardo, the bronze horse that has a long and interesting history within Kosice (I am not entirely sure of the whole story so will cover this another time when i know!).

Chris with Ardo the bronze horseMy first week of lectures in vet school is now over, its been a week of early starts, late finishes and yet I love it and have never been happier! This weekend is now for revision, and more importantly I need to really work on my diary and letter writting to try and raise the £4000 that I need to continue studying here in February!